In vitro and in vivo antiplasmodial activities of gynura. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Antimicrobial activity of single and combined extracts of. The europeans administered an infusion of the plant for convulsions and the hottentots used an infusion of the flower and leaf for typhoid and other fevers. Antimicrobial activity of crude extracts of three medicinal. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. In vitro antibacterial activity of several plant extracts. The extract prolonged survival time of mice at all dose levels which is associated with. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material.
Empirical analysis of the crop biotechnology innovation market methodology. It is likely that specific terpenoids are present in all these plants studied by other workers to show their antimalarial activities. In vitro antiplasmodial activity of extracts and fractions. Antibacterial activity of plant extracts and phytochemicals. The current study investigated the antimicrobial activity, toxicity and. Chapter 4 antiplasmodial activity of oncosiphon piluliferum. Although the mechanism of action of the antiplasmodial activities of the extract has not been investigated, the presence of. The in vitro antiplasmodial activity and cytotoxicity of extracts from jatropha tanjorensis leaves was evaluated. Both plant extracts also demonstrated antiplasmodial activity against the chloroquine resistant plasmodial parasites. Herbs, spices, plant extracts and essential oils eo have been used for centuries to preserve food for humans and to make it more appetizing. Evaluation of the antiplasmodial properties of selected. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus. Screening of antibacterial activity of the medicinal plant. The antiplasmodial activities of the plant extracts when administered twice daily were not significantly different p 0.
Microbial infections till date remain a scourge of humanity due to lack of vaccine against some infections, emergence of drug resistant phenotypes, and the resurgence of infections amongst others. The present study is to evaluate in vitro antiplasmodial activity of extracts from kenyan plants commonly used by traditional healers for the treatment of malaria and other diseases. Pdf merge combinejoin pdf files online for free soda pdf. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Antimicrobial activity, toxicity and phytochemical screening. None of the aqueous extracts showed antimicrobial activity. All plant samples displayed various antiplasmodial activity. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmodial activity against the d6 plasmodium falciparum strain, while only three meoh extracts were active against the w2 strain. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays, chromatographic techniques such as hplc and, tlc as well as nonchromatographic techniques such as immunoassay and fourier transform infra red ftir are discussed. The present study involves in vitro evaluation of two medicinally important plants aristolochia griffithii and thalictrum foliolosum dc used in. A free and open source software to merge, split, rotate and extract pages from pdf files. Biochemistry graduates to have results from their final year research projects on the antibacterial activity of plant extracts submitted for publication dilruba meah and aiysha thompson have just finished their biochemistry. The plant parts namely, leaf, bark, and seeds were thoroughly washed and shadedried.
Or extract pages from a pdf to create a brandnew document. Antibacterial activities of some borneo plant extracts. The thresholds for the in vitro antiplasmodial activity of the plant extracts fractions were based on the classification according to gessler et al. In vitro antiplasmodial activity of callus culture. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity.
Qualitative test revealed the presence of alkaloids in the leaves of p. Antiplasmodial compounds from leaves of dodonaea angustifolia. Anurag sharma and rakesh gupta abstract in the present study, aqueous extract of eight plants, namely azadirachta indica a. Phytochemical investigation and biological activity of leaves. The plant extracts were tested for in vitro activity against a. In vitro antiplasmodial activity and cytotoxicity of extracts and fractions of vitex madiensis, medicinal plant of gabon. Biochemistry graduates to have results from their final year. The antiplasmodial activity 50% inhibitory concentration, ic50 of plant extracts ranged from 1. The aim of this study was to evaluate the antibacterial activity of 28 plant extracts and oils. Total phenolic content, in vitro antioxidant activity and. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material.
Issn phytochemical and antimicrobial evaluation of plant. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. A good correlation between the in vitro and in vivo antiplasmodial activity of these selected medicinal plant species exists. A mean survival time of 19 days observed in the root extract supported its antiplasmodial activities compared with other plant parts. Studies on some plant extracts for their antimicrobial. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. Antiplasmodial activity of vernonia adoensis aqueous. The antiplasmodial activity of 80 % methanol extracts of the plants were assessed in vivo using swiss albino mice against chloroquine sensitive rodent malaria parasite, plasmodium berghei, using the standard 4day suppressive test procedure at doses of 200,400 and 800 mgkgday. The present study reports on the in vitro antiplasmodial activity of 4 plant taxa native to or naturalised in south africa, representing 54 families, which were selected semiquantitatively using weighted criteria. This online pdf merger allows you to quickly combine multiple pdf files into one pdf document for free. Antibacterial activity of some medicinal plant extracts.
Seven of the eleven extracts exhibited significant antiplasmodial activity ic 50 edulis, c. At lower concentrations, trophozoites and schizones persisted with defects in morphological conditions. Resistance development in human malaria parasites against commonly used antimalarial drugs has necessitated the scientific exploration of traditionally used antimalarial plants. The major phytochemical constituents of the plant are elephantopin, triterpenes, stigmasterol, epofriedelinol and lupeol 11,12. The plant extracts were tested for antifungal and antibacterial activities against 4 yeasts and 4 bacteria. Ajuga remota possesses compounds such as, terpenoids, sterols, flavonoids and saponins that might be responsible for its antiplasmodial activity 8,9,10. In vitro antibacterial activity of several plant extracts and. Sorbitolsynchronized parasites were incubated under normal culture conditions at 2% hematocrit and 1% parasitemia in the absence or presence of increasing concentrations of plant extracts. Antiplasmodial activity of some medicinal plants used in. By ngaruiya paul njuguna a thesis submitted in partial fulfilment of master of.
These activities could be attributed to some secondary metabolites of this plant which have been reported to have antiplasmodial activity with varying mechanisms of action. For each species, nhexane, dichloromethane, and methanol extracts were evaluated on a. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. Of the 3 plant extracts tested, the highest antiplasmodial ic50 of 8. Pdf antibacterial activity of medicinal plant extracts. Antiplasmodial activity an overview sciencedirect topics.
Of the 4 species assayed, 49% showed promising antiplasmodial activity ic50. Antimicrobial activity of crude extracts of some plant leaves. Chemical composition and antibacterial activity of essential. Fresh leaves of eleven different plant species free from. The in vitro antiplasmodial activities of 69 alkaloids from various strychnos species were evaluated against chloroquineresistant and chloroquinesensitive lines of plasmodium falciparum. In vitro antiplasmodial activity of crude extracts from togolese medicinal plants by koffi koudouvo, simplice d. Materials and methods plant material elephantopus scaber linn. Evaluation of in vivo antiplasmodial activities of. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmo. Gentle heart puncture from donor mouse and anesthetized with chloroform afforded 1 ml blood the rising. The plant extracts company the plant extracts company pty ltd.
Antibacterial activity of some leaf extracts against xanthomonas campestris pv. In vitro antiplasmodial activity of crude extracts from. Antimicrobial activity of some plant extracts against. Phytochemical screening of plant extracts especially those which have been used in traditional medicine is therefore essential so as to identify phytochemical constituents in the plants that are responsible for a given bioactivity. Antibacterial activity of plants 719 effective extracts are considered, e. Pdf in vitro antiplasmodial activity and cytotoxicity of. Antiplasmodium falciparum activity of extracts from 10. Contrerasesquivel a raul rodriguezherrera a maria l. Total phenolic content, in vitro antioxidant activity and chemical composition of plant extracts from semiarid mexican region author links open overlay panel jorge e. In vitro antiplasmodial activity of flower extracts from. Antiplasmodial activity of medicinal plant preparations. According to the results, the methanol extract of aerial part of p.
In vitro antimalarial activity and cytotoxicity of. Surveys have been conducted and plants have been selected to assess their antimalarial properties through in vitro and in vivo biological assays. The investigation of antimicrobial activity as well as cell toxicity of extracts from 30 plant species against five bacteria species and two fungi species was studied 29. Five grams of each ethanolic extract was separately dissolved in 50 ml distilled water and exhaustively extracted with petroleum ether which was evaporated in vacuo to give semidried extracts denoted as extract. The selected plants, acokanthera oppositifolia lam apocynaceae is a species encountered in eastern. For extracts of cassia occidentalis leaves, morinda morindoides leaves and phyllanthus niruri whole plant, the in vivo antiplasmodial activity has already been reported tona et al. In vitro antimalarial activity and cytotoxicity of some selected cuban medicinal plants. Methanol, ethylacetate extracts of plant species heracleum crenatifolium, h. Pdf evaluation of antiplasmodial activity and safety of. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material. Evaluation of the antiplasmodial activity of extracts of. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibioticresistant bacteria. Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1.
The assessment of the in vitro antiplasmodial activity of cassia sieberiana leaves extracts showed a very good activity 100% of plasmodium elimination 4. Materials and methods plant materials the plant materials were collected from western ghats of karnataka india. Phytochemical analysis of phyllanthus amarus extracts s. Department of chemistry, college of natural sciences, can tho university. Pdf antiplasmodial activity and phytochemical analysis. The plant extracts were tested for in vitro activity against a plasmodium falciparum strain d10 using the parasite lactate dehydrogenase pldh assay. Phytochemical screening and antiplasmodial sensitivity. Membrane stabilizing activity of xanthium strumarium leaves. Cytotoxic effects of active extracts and compounds were assessed. Aqueous, saline buffer and acid extracts of different plant species were screened in vitro for their antifungal activity against alternaria spp. Soda pdf merge tool allows you to combine two or more documents into a single pdf file for free. Here we i assessed the effects of extracts hydrolates of some medicinal plants on pathogenic microorganisms and ii evaluated. Evaluation of antiplasmodial activity and safety of flower extracts of chrysanthemum cinerariaefolium, singly and in combination with chloroquine, lumefantrine and piperaquine. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384.
The invitro studies of methanolic extracts of the selected medicinal plant species on ascaris suum and the three species of bacteria were conducted as a preliminary proof to. Antiplasmodial activity of methanolic leaf extract of. Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants article pdf available in journal of ethnopharmacology 186 march 2016 with 710 reads. Continuous quest for novel therapeutic approaches remains imperative. However, if a previous study revealed that bauhinia rufescens is used in some recipes against malaria, this in vitro study demonstrated that there is no significant antiplasmodial activity in any extract of this plant. Interestingly, they found six extract pairs with apparent synergistic interactions with antiplasmodial activities 5fold that of nefang or the respective extract activities when tested alone. Antibacterial activity of hot aqueous and methanolic extracts prepared from six plants terminallia chebula, terminallia bellerica, phyllanthus emblica, punica granatum, lawsonia alba and mikania micrantha used in traditional folk medicines of india were screened against five pathogenic bacteria staphylococcus aureus mtcc 2940, bacillus subtilis mtcc 441, escherichia coli mtcc 739, proteus.
The plant leaves were successively extracted into three 3 extract forms aqueous extract, ethanolic extract and hydroethanolic 50. Papdiw al international journal of plant protection april, 2010, vol. Various extracts obtained from 19 plants collected in kenya were tested for antiplasmodial activity. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Power your natural product development with the force of nature. Antiplasmodial activity of extracts of selected medicinal. The activity against 15 isolates of each bacterium was determined by disc diffusion method at a concentration of 5%. In the in vivo evaluation of the antiplasmodial activity of the plant extracts was employed against chloroquine cq sensitive p.
Only 22% of these extracts exhibited very high in vitro antiplasmodial activity. Eleven crude extracts prepared from seven different plant species from western cameroon were screened in vitro for their antiplasmodial activity and cytotoxicity. The compounds, comprising mainly indolomonoterpenoid alkaloids, exhibited a wide range of biological potencies in the antiplasmodial assays. Of the 16 extracts tested, 9 showed significant antiplasmodial activity at concentrations less than 50. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Extracts were subjected to in vitro antiplasmodial screening against wild strains of plasmodium falciparum using the nitro.
Sergeyerbanga, 2,3 benjaminkoama, 4 aboubakarsoma, 1. In vitro antiplasmodial activity of flower extracts from combretum leprosum mart mofumbo malaria is the cause of hundreds of deaths per year, besides putting billions of people at risk of developing disease. Such results were different from data reported in literature. The purpose of this study is to investigate the effect of different types of solvent with 50%, 70% and 100% concentration of each solvent on extraction of phenolic compounds from cosmos caudatus. The objective of this study was to determine the chemical composition and antibacterial activity of essential oils eos of tagetes minuta against three phytopathogenic bacteria pseudomonas savastanoi pv. Antimicrobial activities of a plethora of medicinal plant. Research article investigating the mechanism of action of. The components showed slight antimicrobial activity ahmed et al. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. The antiplasmodial activity 50% inhibitory concentration, ic 50 of plant extracts ranged from 1.
Materials and methods plantextracts candidate plants table 1 were selected based on. Antibacterial and antifungal activities from leaf extracts of. Plant material whole plant and fresh apical stems of phyllanthus niruri. Experimental, in vitro, evaluation of the activities of 28 plant extracts and oils as well as some antibiotics against e. It is interesting to note that the action of the extracts of enicostemma hyssopifolium is nontoxic. Salihu no static citation data no static citation data cite.
Bioactivities of extracts and isolation of compounds from tridax procumbens l. Antibacterial characheristics of plants from heracleum genus have relatively slightly been researched. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Antibacterial activities of some plant extracts utilized in. Assessment of antioxidant activity of plant extracts by. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. The extract and its fractions exerted significant reduction of parasitaemia in prophylactic, suppressive and curative models in a dosedependent fashion. These products are well recognized for their health benefits in many societies around the world. Cq and artemisinin were used as positive controls, while 0. Bioactivities of extracts and isolation of compounds from. The structures of isolated compounds were elucidated using chemical and spectroscopic methods. Ten plants indigenous to sudan and of common use in sudanese folkmedicine, were examined in vitro for antimalarial activity against schizonts maturation of plasmodium falciparum, the major human malaria parasite. Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria article pdf available in brazilian journal of microbiology 314 october 2000 with 1,694 reads. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a.
An innovation market approach to analyzing impacts of mergers. Antimicrobial activity of different extracts of pongamia pinnata. It was concluded that ethanol extracts from 70 % of the plants were toxic to cell and only one of the species of combretum duarteanum showed antimicrobial activity. Extraction, isolation and characterization of bioactive.
Gentle heart puncture from donor mouse and anesthetized with chloroform afforded 1 ml blood the rising parasitaemia being about 33%. The presence of the alkaloids in the extract could then explain the moderate antiplasmodial activity observed. Did you know you could combine pdfs to create a new document. This extract was then selected for antiplasmodial activity guided fractionation and isolation of active fractions.
Pdf antiplasmodium falciparum activity of extracts from. Among the various extracts, the lowest 50% inhibitory concentrations ic50 of 24. Inasmuch as plant extracts singly or in combination have been increasingly evaluated for their in vitro antiplasmodial activities, our study is the first to demonstrate the in vitro antiplasmodial activity of nefang and its component plants singly and in paired combinations using both cqsensitive and mdr plasmodium parasites. Plant derivatives have been used for curing malaria historically. In vitro antiplasmodial activity and phytochemical. In vivo antiplasmodial activity of two sahelian plant. Antimicrobial activity of ethanol leaf extracts of. Biological activity of some plant extracts against pieris. In vitro antiplasmodial activities and synergistic.
The results of these investigation showed that plant extracts of enicostemma hyssopifolium possess appreciable and potential antimicrobial activity against commonly encountered microorganisms in humans. The antiplasmodial activity of the extracts and pure compounds was evaluated using the standard peters fourday suppressive method. Effect of combined extracts from different plant parts. A free, open source, platform independent software designed to split, merge, mix, extract pages and rotate pdf files. In vivo and in vitro antimalarial activity of vernonia. Results of antimicrobial activity of the five plant extracts can suggested that s. In vitro antiplasmodial activity of medicinal plants. The mean survival time is important to evaluate the antimalarial activity of plant extracts. Antiplasmodial, antiinflammatory and cytotoxic activities of various plant extracts from the mascarene archipelago. The effect of various plant extracts was measured on several important oxidation indicators. In vitro antiplasmodial activity of central american. Antiplasmodial, antiinflammatory and cytotoxic activities. The chemical examination follows after the isolation and biological screening of the active fraction1. Antiplasmodial activity of solvent fractions of methanolic.
Antibacterial activity of some leaf extracts against. Conclusion from the above studies, it could be concluded that, the methanol extract of plant showed the increase in the protection of the erythrocyte membrane against hypotonic hemolysis which could be reason for its use in table1. Pdf antiplasmodial activity of aqueous leaf extract of. Antibacterial activity of aqueous extract of calotropis. Antiplasmodial potential of medicinal plant extracts from. Access the pdf merger from any internetconnected desktop or mobile device. Biological activity of some plant extracts against pieris brassicae linn.